Detection of protein-cofactor interactions by means of Fourier transform infrared spectroscopy
نویسنده
چکیده
Fourier transform infrared (FTIR) spectroscopy was used to study protein-cofactor interactions in photosystem II. The FTIR spectrum of each cofactor was measured as a difference spectrum upon its light-induced reaction. The spectrum included the bands of the protein moiety coupled to the cofactor, which were identified using selective isotopic labeling of amino acids. Analysis of these FTIR bands provided insight into the protein-cofactor interaction as well as enzymatic reactions. T. Noguchi
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